Silymarin-loaded cellulose acetate electrospun fiber mats: In vitro enzymatic degradability and cytotoxicity tests
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Author list: Phiriyawirut M., Phachamud T.
Publication year: 2012
Journal: Research Journal of Pharmaceutical, Biological and Chemical Sciences (0975-8585)
Volume number: 3
Issue number: 2
Start page: 432
End page: 445
Number of pages: 14
ISSN: 0975-8585
Languages: English-Great Britain (EN-GB)
Abstract
Silymarin has been widely used as a hepatoprotectant and in supportive treatment of patients with chronic inflammatory liver disorders. Recently, silymarin can be used to protect the skin from oxidative stress induced by ultraviolet (UV) irradiation.In this research, the silymarin-loaded electrospun cellulose acetate (CA) fibers were prepared which silymarin was added in various amounts (i.e., 2.5-20 wt. % based on the weight of CA powder). The biodegradability of prepared 7.5% w/w silymarin-loaded electrospun CA fibers was conducted in phosphate buffer pH 7.4, collagenase type I solution and lysozyme solution. The biodegradability was measured at 2, 4, 6 and 8 weeks. The results found that the CA fiber and silymarin-loaded electrospun CA fibers were not degraded by these test solutions. SEM study of tested fibers after drying with lyophilization technique confirmed the durability of these fibers. Cytotoxicity to human fibroblast cell of the prepared fibers was examined by XTT assay for cell viability value. The human fibroblast cell viability after exposure with 0%, 2.5%, 10%, 20% silymarin-loaded electrospun CA fibers and control was 53.10 ฑ 2.24%, 64.29 ฑ 15.36%, 78.01 ฑ 5.92%, 64.14 ฑ 5.15%, and 100% respectively. The percentage of cell viability was lower as the silymarin loading was increased. This result implied that fibroblast cell was sensitive with both CA and silymarin.
Keywords
cytotoxicity, Degradability
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