Infection and colonization of tissues of the aphid Myzus persicae and cassava mealybug Phenacoccus manihoti by the fungus Beauveria bassiana
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Author list: Amnuaykanjanasin A., Jirakkakul J., Panyasiri C., Panyarakkit P., Nounurai P., Chantasingh D., Eurwilaichitr L., Cheevadhanarak S., Tanticharoen M.
Publisher: Springer
Publication year: 2013
Journal: BioControl (1386-6141)
Volume number: 58
Issue number: 3
Start page: 379
End page: 391
Number of pages: 13
ISSN: 1386-6141
eISSN: 1573-8248
Languages: English-Great Britain (EN-GB)
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Abstract
Histopathogenesis of living insects of Myzus persicae Sulzer (Hemiptera: Aphididae) and Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) by Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) was monitored from penetration through insect death. Important events in aphids included fungal penetration of the integument of the less-resistant leg intersegmental membrane and invasion of natural openings, formation of hyphal bodies in live aphids by three days post-inoculation (PI), and extensive hyphal colonization of the two leg segments closest to the insect body at death of the aphids. Confocal microscopy of green fluorescent protein-labeled B. bassiana in live mealybugs indicated the fungus penetrated the host through the legs and mouthparts. The fungus was scarce in live mealybugs at 1-5 days PI, formed hyphal bodies by six days PI, and growth was limited to parts of dead hosts at 6-7 days PI. In dead mealybugs, hyphal bodies were near solid tissue. Blastospores were in the hemolymph. ฉ 2012 International Organization for Biological Control (IOBC).
Keywords
Beauveria bassiana (Ascomycota: Hypocreales), Confocal laser scanning microscopy, Electron microscopy, Entomopathogen, Green fluorescent protein, Insect pathogenesis
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