Subcellular localization-dependent regulation of the three Spirulina desaturase genes, desC, desA, and desD, under different growth phases
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Mapaisansup T., Yutthanasirikul R., Hongsthong A., Tanticharoen M., Ruengjitchatchawalya M.
Publisher: Springer
Publication year: 2013
Journal: Journal of Applied Phycology (0921-8971)
Volume number: 25
Issue number: 2
Start page: 467
End page: 475
Number of pages: 9
ISSN: 0921-8971
eISSN: 1573-5176
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Spirulina, a well-known cyanobacterium, is a potential alternative source for commercial γ-linolenic acid (C18:3Δ9,12,6, GLA) production. During the Spirulina desaturation process, three enzymes, which are encoded by desC, desA, and desD, respectively, introduce double bonds at the Δ9, Δ12, and Δ6 positions of stearic acid (C18:0), oleic acid (C18:1Δ9), and linoleic acid (C18:2Δ9,12). In the present study, transcriptional and translational expression of the desaturase genes during various growth phases of Spirulina platensis Z19/2 was examined. Moreover, the desaturase levels and fatty acids were analyzed in two subcellular locations, the plasma membrane and thylakoid membrane. The results obtained in this study indicated three important points: (1) the regulation level of each Spirulina desaturase gene is possibly subcellular location dependent; (2) GLA is important during cell division in the mid-log phase; and (3) vaccenic acid (C18:1Δ11), which is detected at high levels during the lag phase in the plasma membrane, might play a role in the mechanical strength of the cell membrane at low growth rates. © 2012 Springer Science+Business Media B.V.
Keywords
Delta 12 desaturase, Delta 6 desaturase, Delta 9 desaturase, Growth phase, Regulation, Spirulina platensis
Contact Information