Sodium dodecyl sulfate-modified electrochemical paper-based analytical device for determination of dopamine levels in biological samples

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Rattanarat P., Dungchai W., Siangproh W., Chailapakul O., Henry C.S.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2012

วารสาร: Analytica Chimica Acta (0003-2670)

Volume number: 744

หน้าแรก: 1

หน้าสุดท้าย: 7

จำนวนหน้า: 7

นอก: 0003-2670

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865552000&doi=10.1016%2fj.aca.2012.07.003&partnerID=40&md5=bcc5d45e8aba776c9bae34e98af90003

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

We report the development of an electrochemical paper-based analytical device (ePAD) for the selective determination of dopamine (DA) in model serum sample. The ePAD device consists of three layers. In the top layer, SU-8 photoresist defines a hydrophilic sample application spot on the filter paper. The middle layer was made from transparency film and contained two holes, one for sample preconcentration and the other for the surfactant to allow transfer to the third layer. A screen-printed carbon electrode formed the bottom layer and was used for electrochemical measurements. In the absence of the anionic surfactant, sodium dodecyl sulfate (SDS), the oxidation peaks of DA, ascorbic acid (AA) and uric acid (UA) overlapped. With the addition of SDS, the DA oxidation peak shifted to more negative values and was clearly distinguishable from AA and UA. The oxidation potential shift was presumably due to preferential electrostatic interactions between the cationic DA and the anionic SDS. Indeed, whilst the SDS-modified paper improved the DA current five-fold, the non-ionic Tween-20 and cationic tetradecyltrimethylammonium bromide surfactants had no effect or reduced the current, respectively. Furthermore, only the SDS-modified paper showed the selective shift in oxidation potential for DA. DA determination was carried out using square-wave voltammetry between -0.2 and 0.8. V vs. Ag/AgCl, and this ePAD was able to detect DA over a linear range of 1-100μM with a detection limit (S/N = 3) of 0.37μM. The ePAD seems suitable as a low cost, easy-to-use, portable device for the selective quantitation of DA in human serum samples. © 2012 Elsevier B.V..

คำสำคัญ

Dopamine, Electrochemical detection, Human serum, Paper-based analytical device, Sodium dodecyl sulfate, Uric acid