Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase
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Author list: Waeonukul R., Kosugi A., Prawitwong P., Deng L., Tachaapaikoon C., Pason P., Ratanakhanokchai K., Saito M., Mori Y.
Publisher: Elsevier
Publication year: 2013
Journal: Bioresource Technology (0960-8524)
Volume number: 130
Start page: 424
End page: 430
Number of pages: 7
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
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Abstract
This report describes a novel recycling method utilizing a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase (CglT). To recover cellulosomes and CglT through re-binding to additional cellulose, a chimeric CBM3-CglT was created by fusing carbohydrate binding module (CBM3) from the scaffolding protein CipA into the N-terminal region of CglT. When a recycling test using cellulosomes and CBM3-CglT was performed on microcrystalline cellulose, the process was capable of 4 rounds of recycling (1%. w/v. cellulose/round). Although irreversible absorption of cellulosomes and CBM3-CglT into the residues was observed when ammonia-pretreated rice straw and delignified rice straw was used as substrates, a maximum of 2 and 4 recycling rounds (1%. w/v. glucan/round) were achieved, respectively, consistent with a 70% saccharification rate. This novel recycling method using cellulosomes and CBM3-CglT has great potential as an effective lignocellulose degradation system. © 2012 Elsevier Ltd.
Keywords
β-Glucosidase
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