Synthesis of antibodies-conjugated fluorescent dye-doped silica nanoparticles for a rapid single step detection of campylobacter jejuni in live poultry
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Author list: Tansub W., Tuitemwong K., Limsuwan P., Theparoonrat S., Tuitemwong P.
Publisher: Hindawi
Publication year: 2012
Journal: Journal of Nanomaterials (1687-4110)
Volume number: 2012
ISSN: 1687-4110
eISSN: 1687-4129
Languages: English-Great Britain (EN-GB)
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Abstract
The preparation of antibodies-conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) was developed to detect Campylobacter jejuni cells under a fluorescence microscope. The particles prepared by sol-gel microemulsion techniques have a round shape with an average size of 43ฑ4nm. They were highly photo stable and could emit strong orange fluorescent for 60min. Both amine- and carboxyl-functionalized properties were evident from FTIR and FT Raman spectra. The FDS-NPs conjugated with antibodies against C. jejuni were well dispersed in PBS solution at 20mM of NaCl. The conjugation with monoclonal antibodies against C. jejuni was successful. The direct observation of the antibodies-conjugated FDS-NPs- that bounds C. jejuni with Petroff Hausser counting chamber at 40x was clear. The different focus lengths clearly separated bound and unbound FDS-NPs under the microscope. We successfully synthesis the bio-conjugated dye doped silica nanoparticles for C. jejuni that are easy to use and giving clear detection in due time. Copyright ฉ 2012 Wachira Tansub et al.
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