Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay

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Authors/Editors

WERASAK SURAREUNGCHAI

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Publication Details

Author list: Punbusayakul N., Talapatra S., Ajayan P.M., Surareungchai W.

Publisher: BioMed Central

Publication year: 2013

Journal: Chemistry Central Journal (1752-153X)

Volume number: 7

Issue number: 1

ISSN: 1752-153X

eISSN: 1752-153X

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878895079&doi=10.1186%2f1752-153X-7-102&partnerID=40&md5=0b0c1a720aaa6308290641db5fdd8dff

Languages: English-Great Britain (EN-GB)

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Abstract

Background: A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting Salmonella typhimurium. The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti-Salmonella impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV vs. Ag/AgCl) and the anti-Salmonella concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV vs. Ag/AgCl) and the optimum impregnated anti-Salmonella of 10 μg/mL to detect S. typhimurium cells (0-109 CFU/mL).Results: The DW immunosensor exhibited a detection range of 102 to 107 CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to S. typhimurium but no current response to Escherichia coli.Conclusions: These findings suggest that the use of a label-free DW immunosensor is promising for detecting S. typhimurium. © 2013 Punbusayakul et al.; licensee Chemistry Central Ltd.

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