A stochastic model correctly predicts changes in budding yeast cell cycle dynamics upon periodic expression of CLN2

Journal article

Authors/Editors

TEERAPHAN LAOMETTACHIT

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Publication Details

Author list: Oguz C., Palmisano A., Laomettachit T., Watson L.T., Baumann W.T., Tyson J.J.

Publisher: Public Library of Science

Publication year: 2014

Journal: PLoS ONE (1932-6203)

Volume number: 9

Issue number: 5

ISSN: 1932-6203

eISSN: 1932-6203

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901207678&doi=10.1371%2fjournal.pone.0096726&partnerID=40&md5=8ce435cd0c4e72be100d0d1e9715a9c5

Languages: English-Great Britain (EN-GB)

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Abstract

In this study, we focus on a recent stochastic budding yeast cell cycle model. First, we estimate the model parameters using extensive data sets: phenotypes of 110 genetic strains, single cell statistics of wild type and cln3 strains. Optimization of stochastic model parameters is achieved by an automated algorithm we recently used for a deterministic cell cycle model. Next, in order to test the predictive ability of the stochastic model, we focus on a recent experimental study in which forced periodic expression of CLN2 cyclin (driven by MET3 promoter in cln3 background) has been used to synchronize budding yeast cell colonies. We demonstrate that the model correctly predicts the experimentally observed synchronization levels and cell cycle statistics of mother and daughter cells under various experimental conditions (numerical data that is not enforced in parameter optimization), in addition to correctly predicting the qualitative changes in size control due to forced CLN2 expression. Our model also generates a novel prediction: under frequent CLN2 expression pulses, G1 phase duration is bimodal among small-born cells. These cells originate from daughters with extended budded periods due to size control during the budded period. This novel prediction and the experimental trends captured by the model illustrate the interplay between cell cycle dynamics, synchronization of cell colonies, and size control in budding yeast. ฉ 2014 Oguz et al.

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