Effect of extraction temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Chaiklahan R., Chirasuwan N., Triratana P., Tia S., Bunnag B.

ผู้เผยแพร่: Springer

ปีที่เผยแพร่ (ค.ศ.): 2014

วารสาร: Biotechnology and Bioprocess Engineering (1226-8372)

Volume number: 19

Issue number: 2

หน้าแรก: 369

หน้าสุดท้าย: 377

จำนวนหน้า: 9

นอก: 1226-8372

eISSN: 1976-3816

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901241770&doi=10.1007%2fs12257-013-0733-2&partnerID=40&md5=f1c670a6eea0321f369a709881e93933

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

The extraction temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid extraction of Spirulina. The polysaccharide concentration was significantly higher when the extraction was performed at 90ฐC than when it was performed at 80, 70, and 50ฐC. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 ื 10-12 at 50ฐC to 3.02 ื 10 -12 m2/sec at 90ฐC. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide extraction were calculated as 7.958 ื 10-9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively. ฉ 2014 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.

คำสำคัญ

diffusion coefficients