Ethanol Production from Desizing Wastewater Using Co-Culture of Bacillus subtilis and Saccharomyces cerevisiae
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Author list: Tantipaibulvut S., Pinisakul A., Rattanachaisit P., Klatin K., Onsriprai B., Boonyaratsiri K.
Publisher: Elsevier
Publication year: 2015
Volume number: 79
Start page: 1001
End page: 1007
Number of pages: 7
ISSN: 1876-6102
eISSN: 1876-6102
Languages: English-Great Britain (EN-GB)
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Abstract
This research studied the production of ethanol from desizing wastewater of dye-bleaching industry by using co-culture of Bacillus subtilis D (B) and Saccharomyces cerevisiae TISTR 5160 (S). This work began with the study of the growth of single culture of B and S in wastewater by varying incubation temperature. The optimum temperature for the growth of B and S was 37 ฐC and 30ฐC, respectively. For co-culture, the order of S inoculation, the inoculum ratio of B to S, shaking effect, incubation temperature after S addition, and the effect of nitrogen sources were studied. The optimum condition for ethanol production of co-culture was shaking at 150 rpm and 37ฐC with the inoculation of B 1 day before S and the inoculum ratio of B to S was 5:10. This gave the ethanol content of 5.8 g/L after 48 h. Addition of nitrogen sources prolonged the period the ethanol production going to the maximum. ฉ 2015 The Authors. Published by Elsevier Ltd.
Keywords
dye-bleaching industry