Differentiation-inducing effect of piperitenone oxide, a fragrant ingredient of spearmint (Mentha spicata), but not carvone and menthol, against human colon cancer cells
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Author list: Nakamura Y., Hasegawa Y., Shirota K., Suetome N., Nakamura T., Chomnawang M.T., Thirapanmethee K., Khuntayaporn P., Boonyaritthongchai P., Wongs-Aree C., Okamoto S., Shigeta T., Matsuo T., Park E.Y., Sato K.
Publisher: Elsevier
Publication year: 2014
Journal: Journal of Functional Foods (1756-4646)
Volume number: 8
Issue number: 1
Start page: 62
End page: 67
Number of pages: 6
ISSN: 1756-4646
Languages: English-Great Britain (EN-GB)
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Abstract
The n-hexane extract from spearmint (Mentha spicata) leaves was purified via a bioassay-guided fractionation scheme, which was based on the induction of differentiation in a RCM-1 human colon cancer cell line. One of the criteria of a differentiation marker is the formation of ducts determined visually using phase contrast microscopy, the most potent fraction contained piperitenone oxide, based on EI-MS, 1H-NMR and 13C-NMR spectra. Previously, the role of piperitenone oxide was considered as an odor producing compound in spearmint cultivars, but this study indicates the potential medical benefits of this compound. The effective dose of piperitenone oxide was lower than that of any differentiation-inducers found in the bioassay. Piperitenone oxide was categorized into monoterpene such as carvone and menthol, which are also familiar ingredients in Mentha genus plants. However, carvone and menthol did not possess the differentiation-inducing effect. ฉ 2014 Elsevier Ltd.
Keywords
Colon, differentiation, Mentha spicata, Terpenoid
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