Mesoporous hybrid membrane based label free electrochemical immunoassay
Conference proceedings article
ผู้เขียน/บรรณาธิการ
กลุ่มสาขาการวิจัยเชิงกลยุทธ์
ไม่พบข้อมูลที่เกี่ยวข้อง
รายละเอียดสำหรับงานพิมพ์
รายชื่อผู้แต่ง: Pamsubsakul A., Rijiravanich P., Surareungchai W.
ผู้เผยแพร่: Hindawi
ปีที่เผยแพร่ (ค.ศ.): 2015
ISBN: 9781479968015
นอก: 0146-9428
eISSN: 1745-4557
ภาษา: English-Great Britain (EN-GB)
บทคัดย่อ
We demonstrated an application of a mesoporous-silica hybrid membrane (MHM) toward label free electrochemical immunoassay. Mesoporous silica oriented parallel in the columnar pores of a commercial anodic alumina membrane was firstly prepared. The hybrid membrane was further functionalized with antibiodies and followed by interacting with pathogenic samples through immunorecognition event The sensing mechanism relies on the pore blocking from bound bacteria. The blockage could be traced by measuring the decrease in differential pulse voltammetric (DPV) current of ferrocenecarboxylic redox indicator. Herein, we are interested in proof of the concept on characterization of the constructed membrane, testing immunoreactivity on the membrane by ELISA and the detection of the target. The results showed the potential advantage of using this composite nanomaterial for enhancing the sensing response in the application of label free immunoassay. ฉ 2014 IEEE.
คำสำคัญ
label-free electrochemical sensor, Mesoporous hybrid membrane