Rubpy dye-doped silica nanoparticles as signal reporter in a dot fluorescence immunoassay strip
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Author list: Thepwiwatjit N., Thattiyaphong A., Limsuwan P., Tuitemwong K., Tuitemwong P.
Publisher: Hindawi
Publication year: 2014
Journal: Journal of Nanomaterials (1687-4110)
Volume number: 2014
ISSN: 1687-4110
eISSN: 1687-4129
Languages: English-Great Britain (EN-GB)
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Abstract
This paper describes an application of Rubpy dye-doped silica nanoparticles (RSNPs) as signal reporter in a dot fluorescence immunoassay strip for rapid screening of Vibrio cholera O1 (VCO1). These nanoparticles have a spherical shape with an average diameter of 45 nm. They appear luminescent orange when excited with a 312 nm UV lamp. Based on the sandwich immunoassay principle, a test strip was made of a nitrocellulose membrane dotted with monoclonal antibodies against VCO1 as analyte capture molecules. After introducing a test sample, followed by polyclonal rabbit anti-VCO1 antibody conjugated RSNPs as detection reporters and one washing step, the presence or absence of the target bacteria could be identified under UV light by naked eyes. A positive sample would signal a bright orange dot on the strip. The proposed assay had a detection limit of 4.3 ื 10 3 cfu/mL and was successfully applied as a rapid screening test for VCO1 in food samples with high sensitivity, specificity, and accuracy. ฉ 2014 Nualrahong Thepwiwatjit et al.
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