Comparative mitochondrial genome analysis of Pythium insidiosum and related oomycete species provides new insights into genetic variation and phylogenetic relationships

Journal article

Authors/Editors

WEERAYUTH KITTICHOTIRAT

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Publication Details

Author list: Tangphatsornruang S., Ruang-areerate P., Sangsrakru D., Rujirawat T., Lohnoo T., Kittichotirat W., Patumcharoenpol P., Grenville-Briggs L.J., Krajaejun T.

Publisher: Elsevier

Publication year: 2016

Journal: Gene (0378-1119)

Volume number: 575

Issue number: 1

Start page: 34

End page: 41

Number of pages: 8

ISSN: 0378-1119

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84947869705&doi=10.1016%2fj.gene.2015.08.036&partnerID=40&md5=a6d202bdd82062f4fa87b7788460935c

Languages: English-Great Britain (EN-GB)

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Abstract

Oomycetes are eukaryotic microorganisms, which are phylogenetically distinct from the true-fungi, which they resemble morphologically. While many oomycetes are pathogenic to plants, Pythium insidiosum is capable of infecting humans and animals. Mitochondrial (mt) genomes are valuable genetic resources for exploring the evolution of eukaryotes. During the course of 454-based nuclear genome sequencing, we identified a complete 54.9. kb mt genome sequence, containing 2 large inverted repeats, from P. insidiosum. It contains 65 different genes (including 2 ribosomal RNA genes, 25 transfer RNA genes and 38 genes encoding NADH dehydrogenases, cytochrome b, cytochrome c oxidases, ATP synthases, and ribosomal proteins). Thirty-nine of the 65 genes have two copies, giving a total of 104 genes. A set of 30 conserved protein-coding genes from the mt genomes of P. insidiosum, 11 other oomycetes, and 2 diatoms (outgroup) were used for phylogenetic analyses. The oomycetes can be classified into 2 phylogenetic groups, in relation to their taxonomic lineages: Saprolegnialean and Peronosporalean. P. insidiosum is more closely related to Pythium ultimum than other oomycetes. In conclusion, the complete mt genome of P. insidiosum was successfully sequenced, assembled, and annotated, providing a useful genetic resource for exploring the biology and evolution of P. insidiosum and other oomycetes. ฉ 2015 Elsevier B.V..

Keywords

Mitochondrial genome, Oomycete