Recombinant baculovirus mediates dsRNA specific to rr2 delivery and its protective efficacy against WSSV infection

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Rattanarojpong T., Khankaew S., Khunrae P., Vanichviriyakit R., Poomputsa K.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2016

วารสาร: Journal of Biotechnology (0168-1656)

Volume number: 229

หน้าแรก: 44

หน้าสุดท้าย: 52

จำนวนหน้า: 9

นอก: 0168-1656

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969674152&doi=10.1016%2fj.jbiotec.2016.05.007&partnerID=40&md5=be654c61626197c7b5f2626ae991475d

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

White spot syndrome virus (WSSV) is a major causative agent in shrimp farming. Consequently, RNAi technology is an effective strategy to prevent WSSV infection in shrimp especially dsRNA targeting to rr2 of WSSV. In an effort to develop dsRNA expression in shrimp for control of WSSV infection, we developed a recombinant baculovirus expressing recombinant VP28 as the gene delivery system to carry a gene encoding dsRNA specific to rr2 for triggering the RNAi process in shrimp. The results showed that the recombinant baculovirus harboring VP28 was able to express VP28 indicated by Western blot with polyclonal antibody specific to VP28. VP28 transcript was detected in shrimp hemocytes after co-culture hemocytes with the recombinant baculovirus displaying VP28. In addition, we found that shrimp injected with the recombinant baculovirus displaying VP28 and encoding dsRNA synthetic gene specific to rr2 (Bac-VP28-dsrr2) showed the lowest cumulative mortality (33%) at 14 days post infection (dpi) when compared to shrimp injected with baculovirus displaying VP28 (Bac-VP28) (64% cumulative mortality) (p < 0.05). According to the results, shrimp injected with Bac-VP28-dsrr2 also showed significantly lower WSSV copies than shrimp injected with Bac-VP28 (p < 0.05) along with the down-regulation of rr2 expression at 1, 3 and 7 dpi. In conclusion, the Bac-VP28-dsrr2 was effective in prevention of WSSV infection. Therefore, the results obtained here can be applied to the prevention of WSSV infection by mixing the recombinant baculovirus with shrimp feed in the future. ฉ 2016 Elsevier B.V.

คำสำคัญ

Baculovirus, RNAi technology, Rr2