Loading of silicon nanoparticle labels with redox mediators for detection of multiple DNA targets within a single voltammetric sweep
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Author list: Cheeveewattanagul N., Rijiravanich P., Surareungchai W., Somasundrum M.
Publisher: Elsevier
Publication year: 2016
Journal: Journal of Electroanalytical Chemistry (1572-6657)
Volume number: 779
Start page: 61
End page: 66
Number of pages: 6
ISSN: 1572-6657
Languages: English-Great Britain (EN-GB)
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Abstract
We have constructed electrochemical labels by loading four different redox mediators into silica nanoparticles (SiNPs) through electrostatic attraction. The mediators were methylene blue, ferrocinium tetrafluoroborate, tris (2,2-bipyridyl) dichlororuthenium (II) hexahydrate and acridine orange. The SiNPs were coated with avidin, which enabled attachment to biotin-tagged DNA sequences, forming signal probes. The immobilisation of four different capture probes onto screen printed electrodes enabled a simple qualitative and semi-quantitative detection of four different target probes within a single voltammetric sweep using differential pulse voltammetry. This was illustrated by detecting sequences found in four forms of the influenza virus, the influenza A sub-types H1N1, H3N2, H5N1 and influenza B. The limits of detection were 1.6 pM, 1.6 pM, 4.7 pM and 2.9 pM, respectively. ฉ 2016
Keywords
Redox dyes, Simultaneous detection
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