Structural features and enzymatic digestibility of Napier grass fibre treated with aqueous ammonia
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Publication Details
Author list: Phitsuwan P., Charupongrat S., Klednark R., Ratanakhanokchai K.
Publisher: Elsevier
Publication year: 2015
Journal: Journal of Industrial and Engineering Chemistry (1226-086X)
Volume number: 32
Start page: 360
End page: 364
Number of pages: 5
ISSN: 1226-086X
Languages: English-Great Britain (EN-GB)
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Abstract
We characterised the structural changes of Napier grass after treatment with aqueous ammonia (27%, w/w) at a solid:liquid ratio of 1:12 for 14 days at ambient temperature and evaluated its enzymatic digestibility. This treatment protected carbohydrates in the fibre and greatly reduced lignin content (58.7% lignin removal), resulting in the deconstruction of the fibrous matrix. Although the relative cellulose content increased, the degree of crystallinity of the treated fibre increased only marginally from 42.9 to 50.0%, which can be attributed to the partial disruption of the cellulose crystal structure. Aqueous ammonia treatment markedly promoted the enzymatic cellulose digestibility, increasing from 18 to 53% with an enzyme loading of 30 FPU/g biomass. Supplementation of cellulase with xylanase (30 FPU cellulase + 100 XU xylanase/g biomass) improved the final extent of hydrolysis and greatly enhanced the final glucose yield, approaching to 80% glucan conversion. ฉ 2015 The Korean Society of Industrial and Engineering Chemistry.
Keywords
Aqueous ammonia treatment
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