Tenellin acts as an iron chelator to prevent iron-generated reactive oxygen species toxicity in the entomopathogenic fungus Beauveria bassiana
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Author list: Jirakkakul J., Cheevadhanarak S., Punya J., Chutrakul C., Senachak J., Buajarern T., Tanticharoen M., Amnuaykanjanasin A.
Publisher: Oxford University Press
Publication year: 2015
Journal: FEMS Microbiology Letters (0378-1097)
Volume number: 362
Issue number: 2
ISSN: 0378-1097
eISSN: 1574-6968
Languages: English-Great Britain (EN-GB)
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Abstract
Iron is an essential element for life. However, the iron overload can be toxic. Here, we investigated the significant increase of tenellin and iron-tenellin complex production in ferricrocin-deficient mutants of Beauveria bassiana. Our chemical analysis indicated that the ferricrocin-deficient mutants T1, T3 and T5 nearly abolished ferricrocin production. In turn, these mutants had significant accumulation of iron-tenellin complex in their mycelia at 247-289 mg g-1 cell dry weight under iron-replete condition. Both tenellin and iron-tenellin complex were not detected in the wild-type under such condition. Mass analysis of the mutants' crude extracts demonstrated that tenellin formed a 3:1 complex with iron in the absence of ferricrocin. The unexpected link between ferricrocin and tenellin biosynthesis in ferricrocin-deficient mutants could be a survival strategy during iron-mediated oxidative stress. ฉ 2014 FEMS.
Keywords
Beauveria bassiana, Ferricrocin, intracellular siderophore, iron chelator, iron-generated reactive oxygen species, tenellin
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