Protein A/G-Based immunochromatographic test for serodiagnosis of pythiosis in human and animal subjects from Asia and Americas

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Intaramat A., Sornprachum T., Chantrathonkul B., Chaisuriya P., Lohnoo T., Yingyong W., Jongruja N., Kumsang Y., Sandee A., Chaiprasert A., Banyong R., Santurio J.M., Grooters A.M., Ratanabanangkoon K., Krajaejun T.

ผู้เผยแพร่: Taylor and Francis Ltd

ปีที่เผยแพร่ (ค.ศ.): 2016

Volume number: 54

Issue number: 6

หน้าแรก: 641

หน้าสุดท้าย: 647

จำนวนหน้า: 7

นอก: 1369-3786

eISSN: 1369-3786

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981730995&doi=10.1093%2fmmy%2fmyw018&partnerID=40&md5=ca86e5ecb54d5f772ec6a10f3ae38ec5

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Pythiosis is a life-threatening infectious disease of both humans and animals living in Asia, Americas, Africa, and parts of Australia and New Zealand. The etiologic pathogen is the fungus-like organism Pythium insidiosum. The disease has high mortality and morbidity rates. Use of antifungal drugs are ineffective against P. insidiosum, leaving radical surgery the main treatment option. Prompt treatment leads to better prognosis of affected individuals, and could be achieved by early and accurate diagnosis. Since pythiosis has been increasingly reported worldwide, there is a need for a rapid, user- friendly, and efficient test that facilitates the diagnosis of the disease. This study aims to develop an immunochromatographic test (ICT), using the bacterial protein A/G, to detect anti-P. insidiosum IgGs in humans and animals, and compare its diagnostic perfor- mance with the established ELISA. Eighty-five serum samples from 28 patients, 24 dogs, 12 horses, 12 rabbits, and 9 cattle with pythiosis, and 143 serum samples from 80 human and 63 animal subjects in a healthy condition, with thalassemia, or with other fungal infections, were recruited for assay evaluation. Detection specificities of ELISA and ICT were 100.0%. While the detection sensitivity of ELISA was 98.8%, that of ICT was 90.6%. Most pythiosis sera, that were falsely read negative by ICT, were weakly positive by ELISA. In conclusion, a protein A/G-based ICT is a rapid, user-friendly, and efficient assay for serodiagnosis of pythiosis in humans and animals. Compared to ELISA, ICT has an equivalent detection specificity and a slightly lower detection sensitivity. ฉ The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved.

คำสำคัญ

Immunochromatography, Serodiagnosis