In silico analysis of mucor circinelloides genome-scale model for enhancing lipid production
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Publication Details
Author list: Klanchui A., Vongsangnak W., Laoteng K., Meechai A.
Publisher: Hindawi
Publication year: 2016
Start page: 14
End page: 18
Number of pages: 5
ISBN: 9781450347945
ISSN: 0146-9428
eISSN: 1745-4557
Languages: English-Great Britain (EN-GB)
Abstract
Mucor circinelloides is an attractive oleaginous filamentous fungus because it can produce high content of triacylglycerol (TAG) rich in ?-linolenic acid (GLA). With the increasing attention in microbial oils, many experiments have been carried out in order to enhance storage lipid production in M. circinelloides. Availabilities of genome sequence, biotechnology and computational tools, and pathway databases have facilitated strain improvement through systems metabolic engineering. Genome-scale metabolic model (GEM) has become a useful tool for guiding the design of metabolic engineering strategies. In this study, the GEM of M. circinelloides, iWV1213, was used to investigate candidate genes and metabolites, which could drive metabolic fluxes for lipid production. To evaluate the metabolic capabilities related to growth and lipid formation of iWV1213, the model was firstly simulated and validated with experimental data. Using FBA and MOMA, a single gene deletion together with FVA were performed to identify candidate essential genes for growth and lipid production. Moreover, a single reaction deletion and shadow price analysis were also applied to determine the candidate essential metabolites. The results showed that the top 20 essential genes were involved in amino acid metabolism, TCA cycle, oxidative phosphorylation, and fatty acid oxidation, while the candidate metabolites were involved in lipid biosynthesis, such as malonyl-CoA and acetyl-CoA. These candidates could potentially be used as targets to guide future metabolic engineering strategies for enhancing TAG and GLA production in M. circinelloides. ฉ 2016 ACM.
Keywords
Lipid Production, Mucor circinelloides
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