In vitro production of Spodoptera exigua multiple nucleopolyhedrovirus with enhanced insecticidal activity using a genotypically defined virus inoculum

บทความในวารสาร


ผู้เขียน/บรรณาธิการ


กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง


รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่งChan K.N., Akepratumchai S., Mekvichitsaeng P., Poomputsa K.

ผู้เผยแพร่Elsevier

ปีที่เผยแพร่ (ค.ศ.)2017

วารสารJournal of Biotechnology (0168-1656)

Volume number259

หน้าแรก19

หน้าสุดท้าย25

จำนวนหน้า7

นอก0168-1656

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85027586087&doi=10.1016%2fj.jbiotec.2017.08.001&partnerID=40&md5=b3203b2d54b4eec094aa9ffdfa526369

ภาษาEnglish-Great Britain (EN-GB)


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บทคัดย่อ

Defective virus accumulations during baculovirus passages in insect cell culture are impediments to large scale baculovirus production. A genotypically defined virus inoculum comprises of stable genotypes was proposed for production of a Thailand isolated SeMNPV in Se-UCR1 insect cells. Targeted genotypes were from wild-type SeMNPV containing naturally mixed genotypes. Plaque assays, PCR screening and XbaI restriction analysis were employed for genotype purification, genotype selection and genome analysis, respectively. A selective marker was pif2 encoded per os infection factor which predominantly deleted, along with the adjacent pif1, in defective viruses. A purified, genetically stable pif2+ (and pif1+) genotype, namely SeThpif2+, was the first tryout. SeThpif2+ occlusion bodies (OBs) possessed insecticidal activity but at lower level than the wild-type. When the SeThpif2+ was co-infected with another purified, genetically stable pif1- (and pif2-) genotype, SeThpif2-, at ratio of 3:1, respectively, mixed genotypes OBs had 2.8 times greater insecticidal activity than the SeThpif2+ alone. Dilution of deleterious PIF1 of SeThpif2+ by the pif1 deletion genotypes, SeThpif2-, was the key for this enhanced activity. A promising approach was described for SeMNPV production in vitro using the virus inoculum whose genotypes compositions were designed to mimic virus interactions in the wild-type, to generate per oral infective baculovirus. ฉ 2017 Elsevier B.V.


คำสำคัญ

A genotypically defined virus inoculumDefective virusInsect cell cultureInsecticidesin vitro productionSpodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)


อัพเดทล่าสุด 2023-27-09 ถึง 07:36