Electrochemical assay of proteolytically active prostate specific antigen based on anodic stripping voltammetry of silver enhanced gold nanoparticle labels
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Author list: Parnsubsakul A., Safitri R.E., Rijiravanich P., Surareungchai W.
Publisher: Elsevier
Publication year: 2017
Journal: Journal of Electroanalytical Chemistry (1572-6657)
Volume number: 785
Start page: 125
End page: 130
Number of pages: 6
ISSN: 1572-6657
Languages: English-Great Britain (EN-GB)
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Abstract
This work demonstrated the determination of proteolytically active prostate specific antigen (paPSA), a potential biomarker for prostate cancer diagnosis, in human serum using a sensitive and low-cost electrochemical sensor. A specifically designed peptide probe was immobilized on the surface of a 96-well plate. The probe could be recognized by paPSA causing cleavage of the peptide, resulting in a decrease in the thiols group remaining on the probe. Gold nanoparticles (AuNPs) were attached to the peptide thiol groups by self-assembly. Hence the amount of AuNPs relates to the length of peptide probe. After cleavage and binding of AuNPs, an amplification step was performed using a silver enhancer solution. The quantity of deposited silver was then measured by differential pulse anodic stripping voltammetry (DPASV) using a disposable screen-printed carbon electrode (SPCE). The signal for paPSA detection was the linear range from 0.1 to 100 ng mL− 1, with a detection limit of 27 pg mL− 1. We also showed that the assay was reliable and has potential for clinical applications. © 2016 Elsevier B.V.
Keywords
paPSA, Screen-printed carbon electrode, Silver enhancement
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