Detection of methamphetamine/amphetamine in human urine based on surface-enhanced Raman spectroscopy and acidulation treatments

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Author list: Nuntawong N., Eiamchai P., Somrang W., Denchitcharoen S., Limwichean S., Horprathum M., Patthanasettakul V., Chaiya S., Leelapojanaporn A., Saiseng S., Pongsethasant P., Chindaudom P.

Publisher: Elsevier

Publication year: 2017

Journal: Sensors and Actuators B: Chemical (0925-4005)

Volume number: 239

Start page: 139

End page: 146

Number of pages: 8

ISSN: 0925-4005

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84982850778&doi=10.1016%2fj.snb.2016.07.129&partnerID=40&md5=9912f26d55d1bdc97970b076d3317fce

Languages: English-Great Britain (EN-GB)

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Abstract

Based on the Raman spectrocopy of the nanostructure-based SERS chips, the trace amount of methamphetamine and its primary metabolite, i.e., amphetamine, were successfully identified from human urine. The SERS substrates were self-assembled vertically-aligned silver nanorods prepared by the magnetron sputtering technique. The matrix spikes of the human urine specimens were prepared to simulate those of the methamphetamine abusers. With the acidulation treatments to the specimen samples, the organic urea-based byproducts were clearly separated. The remaining dissolved methamphetamine/amphetamine in the urine specimens could therefore be enhanced for the SERS analyses, without any interference from the urea. The performance of the proposed acidulation treatments toward the SERS detections of the matrix-spike human urine specimens yielded the detection limit at 50 ng/mL. ฉ 2016 Elsevier B.V.

Keywords

Amphetamine, Methamphetamine, Nitric acid, SERS, Silver nanorods, Trace detection