Molecular dynamics exploration of poration and leaking caused by Kalata B1 in HIV-infected cell membrane compared to host and HIV membranes
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Author list: Nawae W., Hannongbua S., Ruengjitchatchawalya M.
Publisher: Nature Research
Publication year: 2017
Journal: Scientific Reports (2045-2322)
Volume number: 7
Issue number: 1
ISSN: 2045-2322
eISSN: 2045-2322
Languages: English-Great Britain (EN-GB)
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Abstract
The membrane disruption activities of kalata B1 (kB1) were investigated using molecular dynamics simulations with membrane models. The models were constructed to mimic the lipid microdomain formation in membranes of HIV particle, HIV-infected cell, and host cell. The differences in the lipid ratios of these membranes caused the formation of liquid ordered (lo) domains of different sizes, which affected the binding and activity of kB1. Stronger kB1 disruptive activity was observed for the membrane with small sized lo domain. Our results show that kB1 causes membrane leaking without bilayer penetration. The membrane poration mechanism involved in the disorganization of the lo domain and in cholesterol inter-leaflet translocation is described. This study enhances our understanding of the membrane activity of kB1, which may be useful for designing novel and potentially therapeutic peptides based on the kB1 framework. ฉ 2017 The Author(s).
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