Overcoming intrinsic restriction enzyme barriers enhances transformation efficiency in arthrospira platensis C1
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Jeamton W., Dulsawat S., Tanticharoen M., Vonshak A., Cheevadhanarak S.
Publisher: Oxford University Press
Publication year: 2017
Journal: Plant & Cell Physiology (0032-0781)
Volume number: 58
Issue number: 4
Start page: 822
End page: 830
Number of pages: 9
ISSN: 0032-0781
eISSN: 1471-9053
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
The development of a reliable genetic transformation system for Arthrospira platensis has been a long-term goal, mainly for those trying either to improve its performance in large-scale cultivation systems or to enhance its value as food and feed additives. However, so far, most of the attempts to develop such a transformation system have had limited success. In this study, an efficient and stable transformation system for A. platensis C1 was successfully developed. Based on electroporation and transposon techniques, exogenous DNA could be transferred to and stably maintained in the A. platensis C1 genome. Most strains of Arthrospira possess strong restriction barriers, hampering the development of a gene transfer system for this group of cyanobacteria. By using a type I restriction inhibitor and liposomes to protect the DNA from nuclease digestion, the transformation efficiency was significantly improved. The transformants were able to grow on a selective medium for more than eight passages, and the transformed DNA could be detected from the stable transformants. We propose that the intrinsic endonuclease enzymes, particularly the type I restriction enzyme, in A. platensis C1 play an important role in the transformation efficiency of this industrial important cyanobacterium. ฉ The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.
Keywords
Liposome, Nuclease, Transformation efficiency
Contact Information