Fungicide Xylaria sp. BCC 1067 extract induces reactive oxygen species and activates multidrug resistance system in Saccharomyces cerevisiae
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Author list: Somboon P., Poonsawad A., Wattanachaisaereekul S., Jensen L.T., Niimi M., Cheevadhanarak S., Soontorngun N.
Publisher: Future Science Group
Publication year: 2017
Journal: Future Microbiology (1746-0913)
Volume number: 12
Issue number: 5
Start page: 417
End page: 440
Number of pages: 24
ISSN: 1746-0913
eISSN: 1746-0921
Languages: English-Great Britain (EN-GB)
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Abstract
Aim: To investigate antifungal potential of Xylaria sp. BIOTEC culture collection (BCC) 1067 extract against the model yeast Saccharomyces cerevisiae. Materials & methods: Antifungal property of extract, reactive oxygen species levels and cell survival were determined, using selected deletion strains. Results: Extract showed promising antifungal effect with minimal inhibitory concentration100 and minimal fungicidal concentration of 500 and 1000 mg/l, respectively. Strong synergy was observed with fractional inhibitory concentration index value of 0.185 for the combination of 60.0 and 0.5 mg/l of extract and ketoconazole, respectively. Extract-induced intracellular reactive oxygen species levels in some oxidant-prone strains and mediated plasma membrane rupture. Antioxidant regulator Yap1, efflux transporter Pdr5 and ascorbate were pivotal to protect S. cerevisiae from extract cytotoxicity. Conclusion: Xylaria sp. BCC 1067 extract is a potentially valuable source of novel antifungals. ฉ 2017 Future Medicine Ltd.
Keywords
antifungals, Drug resistance, Natural product, oxidative stress response, Pdr5, ROS, S. cerevisiae, Xylaria, Yap1
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