An improved genome-scale metabolic model of arthrospira platensis C1 (iAK888) and its application in glycogen overproduction

บทความในวารสาร


ผู้เขียน/บรรณาธิการ


กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง


รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่งKlanchui A., Dulsawat S., Chaloemngam K., Cheevadhanarak S., Prommeenate P., Meechai A.

ผู้เผยแพร่MDPI AG

ปีที่เผยแพร่ (ค.ศ.)2018

Volume number8

Issue number4

นอก2218-1989

eISSN2218-1989

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060049614&doi=10.3390%2fmetabo8040084&partnerID=40&md5=c63c91ccc9c73e9d5cb3b46453f08a0b

ภาษาEnglish-Great Britain (EN-GB)


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บทคัดย่อ

Glycogen-enriched biomass of Arthrospira platensis has increasingly gained attention as a source for bioethanol production. To study the metabolic capabilities of glycogen production in A. platensis C1, a genome-scale metabolic model (GEM) could be a useful tool for predicting cellular behavior and suggesting strategies for glycogen overproduction. New experimentally validated GEM of A. platensis C1 namely iAK888, which has improved metabolic coverage and functionality was employed in this research. The iAK888 is a fully functional compartmentalized GEM consisting of 888 genes, 1,096 reactions, and 994 metabolites. This model was demonstrated to reasonably predict growth and glycogen fluxes under different growth conditions. In addition, iAK888 was further employed to predict the effect of deficiencies of NO3 −, PO4 3−, or SO4 2− on the growth and glycogen production in A. platensis C1. The simulation results showed that these nutrient limitations led to a decrease in growth flux and an increase in glycogen flux. The experiment of A. platensis C1 confirmed the enhancement of glycogen fluxes after the cells being transferred from normal Zarrouk’s medium to either NO3 −, PO4 3−, or SO4 2−-free Zarrouk’s media. Therefore, iAK888 could be served as a predictive model for glycogen overproduction and a valuable multidisciplinary tool for further studies of this important academic and industrial organism. © 2018, MDPI AG. All rights reserved.


คำสำคัญ

Arthrospira platensis C1Glycogen


อัพเดทล่าสุด 2023-06-10 ถึง 10:04