Trypsin Hydrolysed Protein Fractions as Radical Scavengers and Anti-bacterial Agents from Ficus deltoidea

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Abdullah F.I., Chua L.S., Rahmat Z., Soontorngun N., Somboon P.

ผู้เผยแพร่: Springer Netherlands

ปีที่เผยแพร่ (ค.ศ.): 2018

Volume number: 24

Issue number: 2

หน้าแรก: 279

หน้าสุดท้าย: 290

จำนวนหน้า: 12

นอก: 1573-3149

eISSN: 1573-3149

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85023204113&doi=10.1007%2fs10989-017-9613-5&partnerID=40&md5=f197522206f195f72a3d843365d07d68

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Different molecular sizes of protein hydrolysates were prepared from the crude protein extract of Ficus deltoidea using the technique of membrane ultrafiltration after trypsin hydrolysis. Gel electrophoretic images shows the presence of 12, 8, 7 and 7 protein bands for the protein fractions prepared from the molecular weight cut-off of 3, 10, 30 and 100 kDa, respectively. The protein hydrolysates were found to have higher radical scavenging activity than those unhydrolysed fractions at the similar molecular size. They exhibited significant differences in the radical scavenging activities based on one-way analysis of variance, except for the protein hydrolysates of 30 and 100 kDa. The smallest protein hydrolysates, 3 kDa appeared to have the comparable activity (30%) with bovine serum albumin as a positive control in this study. Similarly, the 3 kDa protein hydrolysates achieved the highest inhibitory activity (87.5%) against Pseudomonas aeruginosa at the concentration of 128 µg/mL. The protein hydrolysates were found to be more effective against gram negative bacteria (P. aeruginosa and Escherichia coli) because of lower minimum inhibitory concentration (MIC) and effective inhibitory concentration at 50% (EC50) than gram positive bacterium (Staphylococcus aureus). Trypsin catalysed hydrolysis seemed to improve the anti-bacterial activity of protein hydrolysates in a bacterial strain dependent manner. The MIC could achieve 1–55 µg/mL at different molecular sizes of protein fractions. Mass spectra matching revealed that 26% of 226 identified proteins belonged to the category of plant defensive proteins in stress management and metal handling. © 2017, Springer Science+Business Media, LLC.

คำสำคัญ

Anti-radical, Ficus deltoidea, Trypsin, Ultrafiltration membrane