Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Chutichetpong P., Cheeveewattanagul N., Srilohasin P., Rijiravanich P., Chaiprasert A., Surareungchai W.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2018

วารสาร: Analytica Chimica Acta (0003-2670)

Volume number: 1025

หน้าแรก: 108

หน้าสุดท้าย: 117

จำนวนหน้า: 10

นอก: 0003-2670

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046832277&doi=10.1016%2fj.aca.2018.04.064&partnerID=40&md5=035b276499deb43581d892ee51b52c41

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Drug susceptibility testing (DST) for Mycobacterium tuberculosis currently faces multiple challenges, including lengthy ineffective standard methods, the expensive cost of molecular tests, and large bulky diagnostic machines. In this work, a disposable MPT64 sensor was developed to rapidly determine drug susceptibility (DS-TB) and multidrug resistant tuberculosis (MDR-TB) using an electrochemical sandwich-immunosensor for the detection of MPT64 as an indicator of Mycobacterium tuberculosis growth. Anti-MPT64 (as capture antibody; cAb) was immobilised on screen printed carbon electrodes to specifically bind with MPT64 target protein. A reporter probe of anti-MPT64 conjugated with horseradish peroxidase (HRP labeled rAb) then completed the sandwich immunocomplex. The current signals were received from the catalytic reaction between 3,3′-5,5′-tetramethylbenzidine (TMB), H2O2 and HRP labeled rAb using the chronoamperometric mode on a portable potentiostat. Increasing MPT64 concentration was taken as an indicator of growth, which was measured by the disposable MPT64 sensor. This sensor shows the possibility of determining DST by comparing the signals of DS-TB and MDR-TB growth in drug-free and drug-containing liquid medium. The time required to identify DS-TB and MDR-TB in pure culture MTB and leftover sputum sediments from patients are 3 days and 4–6 days, respectively. Therefore, using this sensor is significantly rapid and inexpensive and has the potential to be used for drug susceptibility testing of tuberculosis in middle to low income countries. © 2018 Elsevier B.V.

คำสำคัญ

Disposable MPT64 sensor, Drug susceptibility test, Electrochemical immunosensor, Multi-drug resistant tuberculosis