Salicylicylphenylacetylene fluorophore mixed with graphene oxide for selective lysozyme detection
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Author list: Niamnont N., Jurat N., Sessomboon N., Boonkitpatarakul K., Sukwattanasinitt M.
Publisher: Elsevier
Publication year: 2018
Journal: Journal of Luminescence (0022-2313)
Volume number: 196
Start page: 201
End page: 206
Number of pages: 6
ISSN: 0022-2313
eISSN: 1872-7883
Languages: English-Great Britain (EN-GB)
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Abstract
A highly selective method of lysozyme detection based on salicylicylphenylacetylene (1) fluorophores and graphene oxide (GO) was successfully developed. The fluorescent emission of 1 was decreased fivefold in the addition of GO (0.6 Abs) due to π-π and hydrophobic interaction between 1 and GO graphitic plane. In the presence of lysozyme (Lys), the non-emissive 1-GO became highly fluorescent as a result of strong interaction of 1 with the cationic Lys preventing the complexation between 1 and GO. The linear dynamic response of fluorescence intensity was observed in the Lys concentration range of 0–80 µg/mL with the limit of detection of 65 ng/mL. This label free technique was developed for quantitative determination of Lys in egg white sample with an analytical accuracy of less than 10% error. © 2017 Elsevier B.V.
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