Genome-Wide Identification of Putative MicroRNAs in Cassava (Manihot esculenta Crantz) and Their Functional Landscape in Cellular Regulation
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Publication Details
Author list: Yawichai A., Kalapanulak S., Thammarongtham C., Saithong T.
Publisher: Future Medicine Ltd.
Publication year: 2019
Volume number: 2019
ISSN: 1757-6180
eISSN: 1757-6180
Languages: English-Great Britain (EN-GB)
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Abstract
Aim: Time-based microfluidic absorption sampling was proposed using cotton fiber-based device made in swab stick. The assay was optimized and compared with conventional pipetted drop sampling using the same device. Materials & methods: Reagents were integrated into cotton fiber device for assessing concentration of analytes by the colorimetric detection method through time-based absorption sampling microfluidic system. All assay parameters were first optimized using conventional pipette-based drop sampling. Results: The color intensity is linear in the relevant concentration range of the analytes. The LOD are 0.189 mM for glucose and 6.56 μM for nitrite, respectively. These values are better than conventional drop sampling. The fiber-containing swab itself functions as sampling, assay and calibration device. Conclusion: Microfluidic cotton fiber-based assay device was fabricated and can determine analyte concentration in artificial salivary samples, colorimetrically, by time-based absorption sampling without the need of complex equipments. © 2019 Newlands Press.
Keywords
Absorption sampling, Fiber-based assay, Microfluidic, Nitrite, Noninvasive diagnostic
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