Photostable methylene blue-loaded silica particles used as label for immunosorbent assay of Salmonella Typhimurium
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Author list: Cheeveewattanagul N., Tien T.T., Rijiravanich P., Surareungchai W., Somasundrum M.
Publisher: Wiley-Blackwell Publishing Ltd
Publication year: 2019
Volume number: 66
Issue number: 5
Start page: 842
End page: 849
Number of pages: 8
ISSN: 0885-4513
eISSN: 0885-4513
Languages: English-Great Britain (EN-GB)
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Abstract
Salmonella Typhimurium is a major cause of food poisoning. To solve the limitations of the routine enzyme linked immunosorbent assay such as laborious assay procedure, lack of long-term enzyme stability, and insufficient sensitivity, we provided a non-enzymatic colorimetric immunosorbent assay platform to overcome these problems. The highly photostable redox dye particles was constructed by silica particles (diameter = 598 ± 14.4 nm) loaded with methylene blue (Si-MB) and applied to be a label for immunoassay of S. Typhimurium. The sandwich assay format involved incubation of an analyte in a microplate wells modified with monoclonal anti-Salmonella, followed by exposure to a polyclonal anti-Salmonella/Si-MB bioconjugate and then measurement of absorbance at 598 nm. The platform had an assay time of 20 min, could detect heat-killed Salmonella with a limit of detection of 48 CFU mL−1, and gave good recoveries in milk. The labels could be stored at 4 °C for 70 days without any deterioration. © 2019 International Union of Biochemistry and Molecular Biology, Inc.
Keywords
high sensitivity, non-enzymatic-immunosorbent assay, sandwich immunoassay, silica particles
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