Osteogenic potential of sol–gel bioactive glasses containing manganese

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

ปาริชาต นฤพนธ์จิรกุล

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Barrioni B.R., Norris E., Li S., Naruphontjirakul P., Jones J.R., Pereira M.M.

ผู้เผยแพร่: Springer New York LLC

ปีที่เผยแพร่ (ค.ศ.): 2019

Volume number: 30

Issue number: 7

นอก: 0957-4530

eISSN: 0957-4530

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069046514&doi=10.1007%2fs10856-019-6288-9&partnerID=40&md5=2dfcc730d1d3de2fd138ba92a4d32f81

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mn-containing BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn2+ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Results show that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration. [Figure not available: see fulltext.]. ฉ 2019, Springer Science+Business Media, LLC, part of Springer Nature.

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