Saccharopolyspora rhizosphaerae sp. nov., an actinomycete isolated from rhizosphere soil in Thailand

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Author list: Intra B., Euanorasetr J., Tak้ A., Inahashi Y., Mori M., Panbangred W., Matsumoto A.

Publisher: Microbiology Society

Publication year: 2019

Journal: International Journal of Systematic and Evolutionary Microbiology (1466-5026)

Volume number: 69

Issue number: 5

Start page: 1299

End page: 1305

Number of pages: 7

ISSN: 1466-5026

eISSN: 1466-5034

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065755154&doi=10.1099%2fijsem.0.003307&partnerID=40&md5=2d157af06ad2b826b4543448ee444078

Languages: English-Great Britain (EN-GB)

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Abstract

A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand. Strain H219T was characterised using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6%), Saccharopolyspora dendranthemae NBRC 108675T (97.5%) and Saccharopolyspora flava NBRC 16345T (97.5%). However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48% between the novel isolate and the above closely related strains. The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid. The diagnostic whole-cell sugars consisted of arabinose and galactose. The cellular fatty acid profile mainly comprised iso-C16: 0, anteiso-C17: 0, iso-C15: 0, and 10-methyl C17: 0. The major menaquinone was MK-9(H4). The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolaminecontaining hydroxylated fatty acids and an unknown phospholipid. The DNA G+C content was 70.6 mol%. Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora. However, strain H219T could be distinguished from closely related strains by several phenotypic properties. Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolyspora rhizosphaerae sp. nov. The type strain is H219T (=TBRC 8564T=NBRC 113388T). ฉ 2019 IUMS.

Keywords

Rhizosphere soil, Saccharopolyspora rhizosphaerae sp. nov.