Dengue NS1 detection in pediatric serum using microfluidic paper-based analytical devices
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Author list: Prabowo, Muhammad Hatta; Chatchen, Supawat; Rijiravanich, Patsamon; Limkittikul, Kriengsak; Surareungchai, Werasak;
Publisher: Springer
Publication year: 2020
Journal: Analytical and Bioanalytical Chemistry (1618-2642)
Volume number: 412
Issue number: 12
Start page: 2915
End page: 2925
Number of pages: 11
ISSN: 1618-2642
eISSN: 1618-2650
Languages: English-Great Britain (EN-GB)
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Abstract
The diagnosis of dengue infection is still a critical factor determining success in the clinical management and treatment of patients. Here, the development of microfluidic paper-based analytical devices (μPADs) utilizing a sandwich immunoassay on wax patterned paper functionalized with anti-dengue NS1 monoclonal antibodies for point-of-care detection of dengue NS1 (DEN-NS1-PAD) is reported. Various assay conditions, including the length of the channel and diluent, were optimized, and the response detected by the naked eye and digitized images within 20–30 min. The DEN-NS1-PAD was successfully tested in the field for detecting dengue NS1 in buffer, cell culture media, and human serum. The limit of detection (LoD) of the DEN-NS1-PAD obtained with the naked eye, scanner, and a smartphone camera was 200, 46.7, and 74.8 ng mL−1, respectively. The repeatability, reproducibility, and stability of the DEN-NS1-PAD were also evaluated. High true specificity and sensitivity in the serum of pediatric patients were observed. These evaluation results confirm that the DEN-NS1-PAD can potentially be used in point-of-care dengue diagnostics, which can significantly impact on the spreading of mosquito-borne diseases, which are likely to become more prevalent with the effects of global warming. [Figure not available: see fulltext.]. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Keywords
Dengue, Microfluidic paper-based analytical devices, NS1
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