Identification of viruses and viroids infecting tomato and pepper plants in vietnam by metatranscriptomics

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Choi, Hoseong; Jo, Yeonhwa; Cho, Won Kyong; Yu, Jisuk; Tran, Phu-Tri; Salaipeth, Lakha; Kwak, Hae-Ryun; Choi, Hong-Soo; Kim, Kook-Hyung;

ผู้เผยแพร่: MDPI

ปีที่เผยแพร่ (ค.ศ.): 2020

Volume number: 21

Issue number: 20

หน้าแรก: 1

หน้าสุดท้าย: 16

จำนวนหน้า: 16

นอก: 1661-6596

eISSN: 1422-0067

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092463106&doi=10.3390%2fijms21207565&partnerID=40&md5=55923eea50cd619ff8e1d1f7aebd182c

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Tomato (Lycopersicum esculentum L.) and pepper (Capsicum annuum L.) plants belonging to the family Solanaceae are cultivated worldwide. The rapid development of next-generation sequencing (NGS) technology facilitates the identification of viruses and viroids infecting plants. In this study, we carried out metatranscriptomics using RNA sequencing followed by bioinformatics analyses to identify viruses and viroids infecting tomato and pepper plants in Vietnam. We prepared a total of 16 libraries, including eight tomato and eight pepper libraries derived from different geographical regions in Vietnam. We identified a total of 602 virus-associated contigs, which were assigned to 18 different virus species belonging to nine different viral genera. We identified 13 different viruses and two viroids infecting tomato plants and 12 viruses and two viroids infecting pepper plants with viruses as dominantly observed pathogens. Our results showed that multiple infection of different viral pathogens was common in both plants. Moreover, geographical region and host plant were two major factors to determine viral populations. Taken together, our results provide the comprehensive overview of viral pathogens infecting two important plants in the family Solanaceae grown in Vietnam. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

คำสำคัญ

Metatranscriptomics, Pepper, Vietnam, Virus