Cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene produces higher polyhydroxybutyrate accumulation under modified nutrients of acetate supplementation and nitrogen-phosphorus starvation

Journal article

Authors/Editors

PANUTDA YODSANG

Strategic Research Themes

Molecular Agriculture (Sustainable agriculture)

Publication Details

Author list: Utharn, Suthira; Yodsang, Panutda; Incharoensakdi, Aran; Jantaro, Saowarath;

Publisher: Elsevier

Publication year: 2021

Journal acronym: Biotechnol. Rep.

Volume number: 31

ISSN: 2215017X

eISSN: 2215-017X

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111526956&doi=10.1016%2fj.btre.2021.e00661&partnerID=40&md5=c8809ac2c5486cbc5f79f3bad19f4f09

Languages: English-Great Britain (EN-GB)

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Abstract

Increased polyhydroxybutyrate production in cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene (Δadc1) is first-timely reported in this study. We constructed the mutant by disrupting adc1 gene encoding arginine decarboxylase, thereby exhibiting a partial blockade of polyamine synthesis. This Δadc1 mutant had a proliferative growth and certain contents of intracellular pigments including chlorophyll a and carotenoids as similar as those of wild type (WT). Highest PHB production was certainly induced by BG11-N-P+A condition in both WT and Δadc1 mutant of about 24.9 %w/DCW at day 9 and 36.1 %w/DCW at day 7 of adaptation time, respectively. Abundant PHB granules were also visualized under both BG11-N-P and BG11-N-P+A conditions. All pha transcript amounts of Δadc1 mutant grown at 7 days-adaptation time were clearly upregulated corresponding to its PHB content under BG11-N-P+A condition. Our finding indicated that this adc1 perturbation is alternatively achieved for PHB production in Synechocystis sp. PCC 6803. © 2021 The Author(s)

Keywords

Adc1 mutant, Nutrient deprivation, Polyhydroxybutyrate, Synechocystis sp. PCC6803