Lignocellulolytic enzyme production from Phanerocheate chrysosporium and Fusarium proliferatum PSA-3 during growth on Napier grass and rice straw

Fungi have gained much research attention as s source of lignocellulolytic enzyme. This rekindles interest because biomass has been projected to be a potential feedstock for the sustainable production of biofuels and biochemicals. Here, agricultural residue, namely Napier grass and rice straw, were used as a feedstock for enzyme production by Phanerochaete chrysosporium ATCC201542 and Fusarium proliferatum PSA-3 through solid-state fermentation. After fermentation, the activity of the secreted enzyme by both fungal species were determined. We found that Napier grass and rice straw likely triggered different lignocellulolytic systems in P. chrysosporium ATCC201542 and F. proliferatum PSA-3. During growth on rice straw, strain ATCC201542 produced a relatively higher activity (28.89 U endoglucanase and 31.16 U xylanase) than its growth on Napier grass. Compared with strain ATCC201542, the crude enzyme from F. proliferatum PSA-3 exhibited higher enzymatic activity (i.e., xylanase, endoglucanase, and exoglucanase). The crude enzyme from rice straw growth culture of strain PSA-3 showed 122.81 U xylanase, 44.14 U endoglucanase, and 1.74 U exoglucanase. This study demonstrates the use of low-cost agricultural biomass to produce lignocellulolytic enzymes from P. chrysosporium ATCC201542 and F. proliferatum PSA-3. The enzyme shows promise in converting carbohydrates in biomass to fermentable sugar to biofuel and biochemical production.