Does warming model the way xenoestrogens impact fish liver mass, cell size and cellularity? Let’s find out using the guppy fish!

Xenoestrogensare ubiquitous in aquatic systems, where they may disturb the fish liver morphophysiology.
Warmer waters have multiple (e.g., metabolic) and inevitable consequences to ectothermic animals.
However, our knowledge of the eventually combined effects of EDCs and temperature is still limited.
Does a warmer temperature shapes the way 17α-ethinylestradiol (EE2) impacts the fish liver structure? Adult male guppy exposed for 45 days to defined conditions. Groups: 26 ºC vs 29 ºC vs EE2 50 ng/L (at 26 ºC vs 29 ºC). Design-based STEREOLOGY in 25 μm thick paraffin sections. Parameters: liver volume, cell & nuclear volume, cell number. EE2 at 29 ºC seems to trigger a decrease of liver mass at the cost of smaller cells with proportionally smaller nuclei. The results support the idea that higher temperatures may promote the effects of xenoestrogens in the liver of fish.