Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan
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Author list: Alam, Ibrar; Lertanantawong, Benchaporn; Sutthibutpong, Thana; Punnakitikashem, Primana; Asanithi, Piyapong;
Publisher: MDPI
Publication year: 2022
Volume number: 12
Issue number: 5
ISSN: 2079-6374
eISSN: 2079-6374
Languages: English-Great Britain (EN-GB)
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Abstract
A tryptophan (Trp) sensor was investigated based on electrochemical impedance spectroscopy (EIS) of a molecularly imprinted polymer on a lysozyme amyloid fibril (MIP-AF). The MIP-AF was composed of aniline as a monomer chemically polymerized in the presence of a Trp template molecule onto the AF surface. After extracting the template molecule, the MIP-AF had cavities with a high affinity for the Trp molecules. The obtained MIP-AF demonstrated rapid Trp adsorption and substantial binding capacity (50 µM mg−1 ). Trp determination was studied using non-Faradaic EIS by drop drying the MIP-AF on the working electrode of a screen-printed electrode. The MIP-AF provided a large linear range (10 pM–80 µM), a low detection limit (8 pM), and high selectivity for Trp determination. Furthermore, the proposed method also indicates that the MIP-AF can be used to determine Trp in real samples such as milk and cancer cell media. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
amyloid fibril
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