Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Kanta Sangpairoj, Rapeewan Settacomkul, Tanapan Siangcham, Krai Meemon, Nakorn Niamnont, Nilubon Sornkaew, Montakang Tamtin, Prasert Sobhon, Pornpun Vivithanaporn

ผู้เผยแพร่: Medknow Publications

ปีที่เผยแพร่ (ค.ศ.): 2022

Volume number: 12

Issue number: 3

หน้าแรก: 132

หน้าสุดท้าย: 140

จำนวนหน้า: 9

นอก: 2221-1691

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127063875&doi=10.4103%2f2221-1691.338922&partnerID=40&md5=816292ea75a257024858dd8cd4b7123f

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic effects of HDHE were analyzed. The expression of molecular markers controlling apoptosis, autophagy, DNA damage, and endoplasmic reticulum (ER) stress was determined. Results: HDHE contains a mixture of fatty acids, mainly hexadecanoic acid. HDHE at a cytotoxic concentration induced apoptotic death of MDA-MB-231 cells through mitochondrial membrane dysfunction, and induction of apoptosis markers, and increased the expression of proteins related to DNA damage response. HDHE also induced the expression of LC-3, a marker of autophagic cell death at a cytotoxic concentration. Moreover, HDHE modulated the expression of ER stress genes. Conclusions: The hexadecanoic acid-enriched extract of Halymenia durvillei promotes apoptosis and autophagy of human triple-negative breast cancer cells. This extract may be further explored as an anticancer agent for triple-negative breast cancer. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.

คำสำคัญ

Autophagy, Endoplasmic reticulum stress, Hexadecanoic acid, Red algae