The Antimicrobial Efficiency Test for Solution of Graphene Oxide and Calcium Lactate Using Pixel Counting Method

This research is a study of the antimicrobial efficacy of graphene oxide and calcium lactate coatings on filter paper, using the Pixel counting method to measure the antimicrobial effectiveness. In this study, the test
solutions used to assess antimicrobial efficacy are divided into three types: graphene oxide solution, calcium lactate solution, and a mixture of graphene oxide and calcium lactate solution. In the experiments, 2x2 centimeter filter papers are immersed in the respective solutions and then dried. Subsequently, they are exposed to UV radiation to kill any microorganisms on the filter paper. Following this step, the filter papers are placed in the air for 20 seconds in order to receive the microorganisms. Lastly, the filter papers that have microorganisms from air are placed on a culture medium and incubated at 37 degrees Celsius for 7 days. The Pixel Counting method is employed to evaluate the effectiveness of this research. The distribution of microorganisms is segmented and converted into black and white images, and then a histogram is used to count the pixels of the black area compared to the total area. The results indicate that microorganisms on the graphene oxide solution are distributed at 12.16%, microorganisms on the calcium lactate solution are distributed at 21.08%, and microorganisms on the mixture of graphene oxide and calcium lactate solution are distributed at 26.23%.