Integrative genome analysis of bacteriocin-producing Lactiplantibacillus pentosus LNP1-39 and its synbiotic role in suppressing food-borne pathogens

Journal article

Authors/Editors

SUKANYA PHUENGJAYAEM

Strategic Research Themes

Smart Healthcare (Strategic Research Themes)

Publication Details

Author list: Jirakanjanasit, T., Choovet, N., Booncharoen, A., Kingkaew, E., Poothong, S., Tanasupawat, S., & Phuengjayaem, S.

Publisher: Springer

Publication year: 2025

Journal: Folia Microbiologica (0015-5632)

ISSN: 0015-5632

eISSN: 1874-9356

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-105009877263&doi=10.1007%2Fs12223-025-01285-1&partnerID=40&md5=4d85608b69c9a0433b0a90c4f06317c0

Languages: English-Great Britain (EN-GB)

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Abstract

Lactic acid bacteria were isolated from traditional Thai-fermented foods. Among these, the strain LNP1-39, closely related to Lactiplantibacillus pentosus, was selected for further study because of its non-pathogenic profile. The bacteriocins produced by L. pentosus LNP1-39 were proteinaceous substances that exhibited strong antimicrobial activity across a wide pH range (pH 2–11; 6400–2400 AU/mL) and thermal stability at 100 °C for 40 min (400 AU/mL). These bacteriocins showed a narrow antimicrobial spectrum, effectively targeting Gram-positive pathogens, such as Kocuria rhizophila MIII, Enterococcus faecalis JCM 5803 T, and Listeria monocytogenes ATCC 19115. Comprehensive safety assessments, including whole-genome analysis and in vitro tests, confirmed a low risk of antibiotic resistance and the absence of virulence factors. Strain LNP1-39 was confirmed to be closely related to L. pentosus DSM 20314 T via digital DNA‒DNA hybridization (dDDH; 75.4%), with average nucleotide identity (ANI) at 96.56% ANIb and 97.22% ANIm values. Additionally, LNP1-39 produces pediocin with notable similarity (76.29% identity to pediocin) and presents low risks for antibiotic-resistance genes or transfer genes while providing antioxidant properties. Strain LNP1-39 survived harsh gastrointestinal tract conditions and exhibited a favorable prebiotic index and positive prebiotic activity score when paired with polydextrose or isomalto-oligosaccharide. These findings support L. pentosus LNP1-39 as potential bacteriocin-producing lactic acid bacteria for further application in food preservation and pathogen control or as a synbiotic. © 2025 Elsevier B.V., All rights reserved.

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