Overexpression of Acetyl-CoA carboxylase gene of mucor rouxii enhanced fatty acid content in hansenula polymorpha
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Publication Details
Author list: Ruenwai R., Cheevadhanarak S., Laoteng K.
Publisher: Springer
Publication year: 2009
Journal: Molecular Biotechnology (1073-6085)
Volume number: 42
Issue number: 3
Start page: 327
End page: 332
Number of pages: 6
ISSN: 1073-6085
eISSN: 1559-0305
Languages: English-Great Britain (EN-GB)
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Abstract
Malonyl-CoA is an essential precursor for fatty acid biosynthesis that is generated from the carboxylation of acetyl-CoA. In this work, a gene coding for acetyl-CoA carboxylase (ACC) was isolated from an oleaginous fungus, Mucor rouxii. According to the amino acid sequence homology and the conserved structural organization of the biotin carboxylase, biotin carboxyl carrier protein, and carboxyl transferase domains, the cloned gene was characterized as a multi-domain ACC1 protein. Interestingly, a 40% increase in the total fatty acid content of the non-oleaginous yeast Hansenula polymorpha was achieved by overexpressing the M. rouxii ACC1. This result demonstrated a significant improvement in the production of fatty acids through genetic modification in this yeast strain. ฉ 2009 Humana Press.
Keywords
Acetyl-CoA carboxylase, Fatty acid synthesis, Methylotrophic yeast
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