Optimization of β-carotene production by Rhodotorula glutinis DM28 in fermented radish brine
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Author list: Malisorn C., Suntornsuk W.
Publisher: Elsevier
Publication year: 2008
Journal: Bioresource Technology (0960-8524)
Volume number: 99
Issue number: 7
Start page: 2281
End page: 2287
Number of pages: 7
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
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Abstract
A face-centered central composite design was applied to optimize a cultivation condition for improved β-carotene production by Rhodotorula glutinis DM28 in a stirred tank reactor using 30 g/l total soluble solid of fermented radish brine as a sole substrate. The experiments were performed with regression models, where temperature, pH and dissolved oxygen were considered as variables. Results showed that an optimum condition for β-carotene production of the yeast was at 30 °C, pH 6 and 80% dissolved oxygen. Under this condition, the yeast yielded 2.7 g/l biomass and the maximum β-carotene of 201 μg/l after 24-h fermentation indicating approximately 15% higher than those under an initial condition (2.3 g/l and 178 μg/l, respectively). © 2007 Elsevier Ltd. All rights reserved.
Keywords
fermented radish brine, Rhodotorula glutinis, statistical experimental design, β-carotene
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