Lipid and fatty acids extraction from the cyanobacterium Spirulina
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Publication Details
Author list: Chaiklahan R., Chirasuwan N., Loha V., Bunnag B.
Publication year: 2008
Journal: ScienceAsia (1513-1874)
Volume number: 34
Issue number: 3
Start page: 299
End page: 305
Number of pages: 7
ISSN: 1513-1874
Languages: English-Great Britain (EN-GB)
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Abstract
Lipid extraction from Spirulina using a single stage extraction at 30 °C showed that a sample-solvent ratio of 1:10 and an extraction time of 120 min gave the highest total fatty acid (TFA) yield, whereas a ratio of 1:5 was suitable for a multistage extraction. An increase in extraction temperature resulted in higher lipid and TFA yields. Increasing the extraction temperature from 30°C to 60°C decreased the extraction time and increased the yield of lipid and TFA in the solvent by approximately 69% and 55%, respectively. In order to increase the extraction yield of the lipid and TFA, multistage cross-current extraction was implemented. Seven-stage extraction at 30°C resulted in 85% TFA recovery, whereas only a three-stage extraction was needed to obtain the same level of TFA at 60 °C. Lipid extracted from Spirulina contained approximately 21% linoleic acid and 18% γ-linolenic acid.
Keywords
lipid, Total fatty acid
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