Reconstruction of starch biosynthesis pathway in cassava using comparative genomic approach
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Author list: Rongsirikul O., Saithong T., Kalapanulak S., Meechai A., Cheevadhanarak S., Netrphan S., Suksangpanomrung M.
Publisher: Springer Verlag (Germany): Computer Proceedings
Publication year: 2010
Volume number: 115 CCIS
Start page: 118
End page: 129
Number of pages: 12
ISBN: 3642167497; 9783642167492
ISSN: 1865-0929
Languages: English-Great Britain (EN-GB)
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Abstract
Cassava is one of the most attractive crops nowadays because it can produce and accumulate large amount of starch in its roots. Cassava starch is widely used as food, feed and raw materials for biochemical industries. Due to the increasing demand of cassava starch, the starch biosynthesis pathway is thus of interest for metabolic engineering, aiming at strain improvement. However, the uncertainties in the metabolic pathway of starch biosynthesis in cassava retard the rate of achievement. Availability of recently released cassava genome motivates us to reconstruct the starch biosynthesis pathway in cassava using comparative genomic approach. Here, nucleotide sequences of the template plants (i.e. Arabidopsis and potato) were compared with the sequence of cassava collected from three sources: Phytozome (genomic sequence), Cassava full-length cDNA and Cassava genome (ESTs) databases. The metabolic pathway of approximately 34 enzymes was constructed, including pathway from sucrose metabolism to amylose and amylopectin synthesis. The resulting pathway is a good initial point toward the complete pathway reconstruction. ฉ 2010 Springer-Verlag Berlin Heidelberg.
Keywords
Comparative genomic approach
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