An efficient treatment for detoxification process of cassava starch by plant cell wall-degrading enzymes
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Author list: Sornyotha S., Kyu K.L., Ratanakhanokchai K.
Publisher: Elsevier
Publication year: 2010
Journal: Journal of Bioscience and Bioengineering (1389-1723)
Volume number: 109
Issue number: 1
Start page: 9
End page: 14
Number of pages: 6
ISSN: 1389-1723
Languages: English-Great Britain (EN-GB)
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Abstract
The objective of this work was to remove linamarin in starch from cassava (Manihot esculenta Crantz cv. KU-50) roots, a high-cyanogen variety by using plant cell wall-degrading enzymes, xylanase and cellulase. The combination of xylanase from Bacillus firmus K-1 and xylanase and cellulase from Paenibacillus curdlanolyticus B-6 at the ratio of 1:9 showed the maximum synergism at 1.8 times for hydrolyzing cassava cortex cell walls and releasing linamarase. Combined enzyme treatment enhanced linamarin liberation from the parenchyma by 90%. In addition, when the combined enzymes were applied for detoxification during cassava starch production, a low-cyanide-product was obtained with decreased linamarin concentration (96%) compared to non-enzyme treated tissues. Based on these results, xylanase and cellulase treatment is a good method for low-cyanide-cassava starch production and could be applied for detoxification of cassava products during processing. ฉ 2009 The Society for Biotechnology, Japan.
Keywords
Detoxification process, Linamarase, Linamarin, Plant cell wall-degrading enzyme, Xylanase and cellulase treatment
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