Influence of 1-methylcyclopropene on pectic enzyme activities, gene expression, and cell wall modification in papaya (Carica papaya cv. 'Sunrise') fruit

Journal article

Authors/Editors

VARIT SRILAONG

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Publication Details

Author list: Krongyut W., Esguerra E.B., Maninang J.S., Sugaya S., Gemma H., Srilaong V., Kanlayanarat S.

Publisher: Taylor and Francis Group

Publication year: 2011

Journal: The Journal of Horticultural Science and Biotechnology (1462-0316)

Volume number: 86

Issue number: 6

Start page: 631

End page: 639

Number of pages: 9

ISSN: 1462-0316

eISSN: 2380-4084

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-80955128574&doi=10.1080%2f14620316.2011.11512815&partnerID=40&md5=68b05607530b808ada5d64050f0904a5

Languages: English-Great Britain (EN-GB)

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Abstract

Sunrise' papaya fruit harvested at two stages of maturity [colour break (< 10% yellow peel colour) and 25% yellow peel colour] were treated with 100 nl l-1 1-methylcyclopropene (1-MCP) to determine its effects on ripening, on the activities and levels of gene expression of polygalacturonase (PG), pectin methyl esterase (PME), and β-galactosidase (β-Gal), and on the degradation of cell wall components. 1-MCP delayed ripening and the onset of the climacteric, although the peak in the respiration rate was almost the same as that in untreated control fruit. Colour-break fruit treated with 1-MCP exhibited a continuous increase in ethylene production, but at a lower rate than in control fruit. Consequently, 1-MCP-treated fruit ripened with a concomitant reduction in firmness, which was accompanied by an increase in PG and β:-Gal enzyme activities and gene expression. On the other hand, fruit treated with 1-MCP at the 25% yellow stage exhibited lower levels of ethylene production and developed pulp with a rubbery texture at the ripe stage which was attributed to reduced PG, β-Gal, and PME enzyme activities and gene expression.This was consistent with the higher level of cell wall polysaccharides measured in 1-MCP-treated fruit. The above results indicated that 'Sunrise' papaya fruit can be treated with 1-MCP at the colour break stage since they have a greater capacity to recover from the effects of 1-MCP than fruit treated at the 25% yellow stage.

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