Characterisation of floral anthocyanins and their antioxidant activity in Vanda hybrid (V. teres x V. hookeriana)
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Junka N., Kanlayanarat S., Buanong M., Wongs-Aree C.
Publisher: WFL Publisher
Publication year: 2012
Journal: Journal of Food, Agriculture and Environment (1459-0255)
Volume number: 10
Issue number: 2
Start page: 221
End page: 226
Number of pages: 6
ISSN: 1459-0255
eISSN: 1459-0263
Languages: English-Great Britain (EN-GB)
View in Web of Science | View citing articles in Web of Science
Abstract
The major anthocyanins and the antioxidant activity of sequentially extracted anthocyanins were evaluated in anthocyanin-based coloured florets of a Vanda hybrid (V. teres x V. hookeriana). Anthocyanins of this Vanda hybrid are less common anthocyanins, the molecules of which showed a bathochromic shift effect and additional peaks of maximum photoabsorption when incubated in two different pH values of 1.0 and 4.5 because of the complexation of a copigment in a molecule. Based on high-performance liquid chromatograph-photodiode array detector (HPLC-PDA) separation, the floral anthocyanins from the Vanda hybrid were only derived from cyanidin. A liquid chromatography-mass spectrometry (LC-ESI-MSn) analysis disclosed 5 major types of cyanidin derivatives were conjugated with hexose sugars and some organic acids that affected the stability of anthocyanin molecules. The extracted anthocyanins from butterfly pea flowers had the lowest molecular change after long storage period showing the delta absorbance (ΔAbs) valued at 0.07 followed by those of Vanda hybrid florets and red grape at 0.12 and 0.20, respectively, after 6 month storage at 25°C in the dark. Modification of conjugating residue patterns of Vanda hybrid anthocyanins was detected by delta E* (ΔE*), which revealed that the saponified anthocyanins had the lowest change at 10.01, while the raw extracted anthocyanins had the highest change at 13.18. The radical- scavenging activity (RSA) of saponified anthocyanin samples was significantly higher than that of extracted and purified samples especially at higher concentrations.
Keywords
Anthocyanin structure, Stabilitity, Vanda hybrid (V. teres x V. hookeriana)
Contact Information